DNA and RNA purification
DNA and RNA extraction is a basic method used in molecular biology. The need for high-quality, highly pure nucleic acid is important for a wide range of research and clinical applications. Nucleic acid purification is an initial step in many molecular biology and genomics workflows.
DNA and RNA samples are often obtained from crude preparations. Genomic DNA, plasmid DNA, and total RNA can be extracted and purified from a variety of sources, including bacterial and mammalian cells, plant tissue, fungal tissue, mammalian tissue, blood, plasma, serum, viruses, buccal swabs, nasals, gel matrices, PCRs, and other enzymatic reactions. Isolation of nucleic acid from these samples often involves lysis of cell membranes or homogenization of the sample, followed by removal of proteins, enzymes, detergents, salts, and lipids.
Common approaches include:
- alkaline extraction
- Phenol-chloroform extraction
- Density gradient centrifugation with caesium chloride (CsCl)
- Oligo(dT)-cellulose chromatography
- silica matrix
- glass beads
- Diatomaceous earth
- Anion exchange chromatography
- Size exclusion chromatography
The final application will dictate the best purification method. Downstream applications include PCR, qPCR, restriction digests, ligation, cloning, genotyping, gene expression analysis, next-generation sequencing (NGS), and Northern and Southern blotting.
Total RNA Extraction Kit
The E.Z.N.A.® Total RNA Kit I provides a simple and rapid method for the isolation of up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues. Multiple samples of up to 1 x 107 eukaryotic cells or 30 mg of tissue can be processed in parallel in less than 20 minutes. The purified RNA can be used in many downstream applications, such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
Fast: 20 minutes or less
Safe: no phenol/chloroform extractions
Versatile: spin and vacuum formats available
High quality – purified RNA suitable for a variety of downstream applications
For research use only. It should not be used in diagnostic procedures.
- Downstream application: PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
- Elution volume: 40-70 μL
- Starting material: Cultured eukaryotic cells and soft tissues
- Initial amount: < 1 x 107 cells or 30 mg of tissue
- RNA Yield: Up to 100 µg
- Processing mode: Manual, spin/vacuum
- Performance: 1 – 24
- RNA Binding Technology: Silica Mini Spin Column
- Binding capacity: 100 µg